Bovine IgG F(ab)2 Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Product Overview

• Article Name: Bovine IgG F(ab)2 Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-601-4304
• Supplier Catalog Number: 601-4304
• Alternative Catalog Number: ROC-601-4304
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Bovine
• Immunogen: Bovine IgG F(ab)2 fragment
• Conjugation: HRP
• Alternative Names: rabbit anti-Bovine IgG F(ab)2 Peroxidase Conjugated Antibody, rabbit anti-Bovine IgG Fab2 HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Bovine
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:40,000 - 1:80,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Bovine IgG F(ab)2 Peroxidase Conjugated Antibody has been tested by ELISA. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as

Images

ELISA Results of Purified Rabbit Anti-Bovine IgG F(ab)2 Antibody Peroxidase Conjugated tested against purified Bovine IgG F(ab)2 HRP. Each well was coated in duplicate with 1.0 µg of Bovine IgG F(ab)2 (p/n 001-0104). The working dilution is 1:69,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).