Bovine IgG F(ab)2 Antibody Alkaline Phosphatase Conjugated, ALP, Rabbit, Polyclonal

Product Overview

• Article Name: Bovine IgG F(ab)2 Antibody Alkaline Phosphatase Conjugated, ALP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-601-4504
• Supplier Catalog Number: 601-4504
• Alternative Catalog Number: ROC-601-4504
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: DOT, ELISA
• Species Reactivity: Bovine
• Immunogen: Bovine IgG F(ab)2 fragment
• Conjugation: ALP
• Alternative Names: rabbit anti-Bovine IgG F(ab)2 alkaline phosphatase Conjugated Antibody, rabbit anti-Bovine IgG Fab2 alk phos Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 0.6 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Bovine
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Bovine IgG F(ab)2 Alkaline Phosphatase Conjugate has been tested by ELISA and dot blot. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 mi

Images

ELISA Results of Purified Rabbit Anti-Bovine IgG F(ab)2 Antibody Alkaline Phosphatase Conjugated tested against purified Bovine IgG F(ab)2 Alk Phos. Each well was coated in duplicate with 1.0 µg of Bovine IgG F(ab)2 (p/n 001-0104). The working dilution is 1:15,200. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase ELISA Substrate (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).