Goat IgG anti-Chicken IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Goat IgG anti-Chicken IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-603-103-002
• Supplier Catalog Number: 603-103-002
• Alternative Catalog Number: ROC-603-103-002
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Gallus
• Immunogen: Anti-Chicken IgG (H&L) was produced by repeated immunization with Chicken IgG whole molecule in goat.
• Conjugation: HRPO
• Alternative Names: Anti-Chicken Antibody, Gt-a-Chicken IgG, Anti-Chicken IgY, gt-a-ch IgY, gt-a-ch IgG, Gt-a-Chicken IgY, Chicken Antibody in Goat, Chicken Secondary Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:10,000, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Chicken IgG (H&L) HRP Conjugated Antibody is tested in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Anti-Chicken IgG HRP Antibody is suitable for IHC. Specific conditions for reactivity and signa

Images

ELISA results of Goat Anti-Chicken IgG Antibody Peroxidase Conjugated tested against purified Chicken IgG protein. Each well was coated in duplicate with 1.0 µg of Chicken IgG (p/n 003-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:97,900. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE-1000.

Western Blot of Goat Anti-Chicken IgG Peroxidase Conjugated Antibody. Lane 1: Opal Prestained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: RFP (p/n 000-001-379) [ 0.5µg]. Lane 3: RFP (p/n 000-001-379) [0.1µg]. Lane 4: HEK293T whole cell lysate spiked RFP [10µg+0.1µg]. Primary Antibody: Rabbit Anti-RFP (p/n 600-401-379) at 1.0µg/mL o/n at 4C. Secondary Antibody: Goat Anti-Chicken IgG HRP (p/n 603-103-002) at 1:40,000 for 30 mins at RT. Blocking: Universal BlockOut Buffer (p/n MB-073) for 30 mins at RT. Predicted MW: ~27kDa.