Chicken IgG (H&L) Secondary Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Chicken IgG (H&L) Secondary Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-603-4602
• Supplier Catalog Number: 603-4602
• Alternative Catalog Number: ROC-603-4602
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Gallus
• Immunogen: Anti-Chicken IgY / IgG whole molecule was produced by repeated immunization with Chicken IgY / IgG whole molecule in rabbit.
• Conjugation: Biotin
• Alternative Names: Rabbit anti-Chicken IgG Biotin Conjugated Antibody, Rabbit anti-Chicken IgG Antibody Biotin Conjugation, Chicken Secondary Antibody, Rabbit anti-Chicken IgY Antibody Biotin
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.5 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:500,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000
• Application Notes: Secondary antibodies are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods

Images

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.