Donkey IgG anti-Chicken IgG (H+L)-unconj., MinX Bo,Go,Gp,Hm,Ho,Hu,Ms,Rb,Rt,Sh, Polyclonal , Unconjugated

Product Overview

• Article Name: Donkey IgG anti-Chicken IgG (H+L)-unconj., MinX Bo,Go,Gp,Hm,Ho,Hu,Ms,Rb,Rt,Sh, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-603-701-C37
• Supplier Catalog Number: 603-701-C37
• Alternative Catalog Number: ROC-603-701-C37
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Gallus
• Immunogen: Chicken IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Donkey anti-Chicken IgG Antibody, Donkey anti-Chicken IgG Pre-adsorbed Antibody, Donkey anti-Chicken IgY Antibody, Donkey anti-Chicken IgY Pre-adsorbed Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.2 by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Chicken IgG (H&L) has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should

Images

ELISA Results of Purified Donkey Anti-Chicken IgG mx10 Antibody tested against purified Chicken IgG (MX Bv, Gt, Gp, Ham, Hs, Hu, Ms, Rb, Rt, Sh). Each well was coated in duplicate with 10 µg of Chicken IgG/IgY (p/n 003-0102) [Red Line], Bovine IgG (p/n 001-0102) [Green Line], Goat IgG (005-0102) [Blue Line], Guinea Pig (p/n 006-0102) [Purple Line], not shown Hamster IgG (p/n 007-0102), Horse IgG (p/n 008-0102), Human IgG (p/n 009-0102), Mouse IgG (p/n 010-0102), Rabbit IgG (p/n 011-0102), Rat IgG (p/n 012-0102), and Sheep IgG (p/n 013-0102). The working dilution is 1:56,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), HRP Conjugate Stabilizer (p/n MB-076), Goat Anti-Donkey IgG HRP conjugated (p/n 616-1302) and TMB substrate (p/n TMBE-1000).

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-701-C37). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-701-C37) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.