Donkey IgG anti-Chicken IgG (H+L)-FITC, MinX Bo,Go,Gp,Hm,Ho,Hu,Ms,Rb,Rt,Sh, Polyclonal

Product Overview

• Article Name: Donkey IgG anti-Chicken IgG (H+L)-FITC, MinX Bo,Go,Gp,Hm,Ho,Hu,Ms,Rb,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-603-702-C37
• Supplier Catalog Number: 603-702-C37
• Alternative Catalog Number: ROC-603-702-C37
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: FLISA,FACS,IF
• Species Reactivity: Gallus
• Immunogen: Chicken IgG whole molecule
• Conjugation: FITC
• Alternative Names: Donkey anti-Chicken IgG Antibody Fluorescein Conjugation, Donkey anti-Chicken IgG FITC Conjugated Antibody, Donkey anti-Chicken IgY Antibody Fluorescein Conjugation, Donkey anti-Chicken IgY FITC
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Anti-Chicken IgG Fluorescein Antibody has been tested by dot blot and western blot. This product is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background le

Images

Western Blot of Fluorescein conjugated Donkey anti-Chicken IgG Pre-adsorbed secondary antibody. Lane 1: Chicken IgG (p/n 003-0102). Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein donkey secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 72 kDa, 25 & 72 kDa for Chicken IgY.

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-701-C37). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-701-C37) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.