Rabbit IgG anti-Goat IgG (F(ab)2)-unconj., MinX none

Product Overview

• Article Name: Rabbit IgG anti-Goat IgG (F(ab)2)-unconj., MinX none
• Biozol Catalog Number: ROC-605-4104
• Supplier Catalog Number: 605-4104
• Alternative Catalog Number: ROC-605-4104
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Goat
• Immunogen: Goat IgG F(ab)2 fragment
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Goat IgG F(ab)2 Antibody, rabbit anti-Goat IgG Fab2 Antibody, rabbit anti-Goat IgG Fab2 fragment
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.6 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Goat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:275,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:25,000
• Application Notes: Anti-Goat IgG F(ab)2 antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

ELISA Results of Rabbit Anti-Goat IgG F(ab)2 Antibody tested against purified Goat IgG F(ab)2. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n 005-0102) [Green Line] and Goat IgG F(ab)2 (p/n 005-0104) [Purple Line]. The working dilution is 1:38,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076) Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125) and TMB substrate (p/n TMBE-1000).