Rabbit IgG anti-Guinea Pig IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Guinea Pig IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-606-4302
• Supplier Catalog Number: 606-4302
• Alternative Catalog Number: ROC-606-4302
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Guinea pig
• Immunogen: Anti-Guinea Pig IgG was produced by repeated immunization with guinea pig IgG whole molecule in rabbit.
• Conjugation: HRPO
• Alternative Names: Guinea Pig IgG (H&L) Antibody, Rb-a-Guinea Pig IgG(H&L) Peroxidase conjugated, Guinea Pig IgG(H&L) Peroxidase Antibody in rabbit, Rabbit IgG (H&L) peroxidase conjugated Secondary Antibody.
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Guinea Pig
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:100,000, IHC: 1:500 - 2,500, WB: 1:2,000 - 10,000
• Application Notes: Anti-Guinea Pig IgG (H&L) peroxidase conjugated has been tested by ELISA and is assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30

Images

Western Blot of Peroxidase conjugated Rabbit Anti-Guinea Pig IgG secondary antibody. Lane 1: Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Guinea Pig IgG. Other band(s): None.