Guinea Pig IgG (H&L) Antibody Alkaline Phosphatase Conjugated, ALP, Rabbit, Polyclonal

Product Overview

• Article Name: Guinea Pig IgG (H&L) Antibody Alkaline Phosphatase Conjugated, ALP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-606-4502
• Supplier Catalog Number: 606-4502
• Alternative Catalog Number: ROC-606-4502
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: DOT, ELISA
• Species Reactivity: Guinea pig
• Immunogen: Guinea Pig IgG whole molecule
• Conjugation: ALP
• Alternative Names: rabbit Anti-Guinea Pig IgG Antibody alkaline phosphatase Conjugation, rabbit Anti-Guinea Pig IgG alk phos Conjugated antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Guinea Pig
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:15,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
• Application Notes: Anti-GUINEA PIG IgG alkaline phosphatase Antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody ba

Images

ELISA Results of Rabbit Anti-Guinea Pig IgG Antibody Alkaline Phosphatase Conjugated tested against purified Guinea Pig IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (p/n 006-0102). The working dilution is 1:14,500. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase ELISA Substrate (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).