Donkey IgG anti-Guinea Pig IgG (H+L)-unconj., MinX Bo,Ck,Go,Hm,Ho,Hu,Ms,Rb,Rt,Sh, Polyclonal , Unconjugated

Product Overview

• Article Name: Donkey IgG anti-Guinea Pig IgG (H+L)-unconj., MinX Bo,Ck,Go,Hm,Ho,Hu,Ms,Rb,Rt,Sh, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-606-701-C36
• Supplier Catalog Number: 606-701-C36
• Alternative Catalog Number: ROC-606-701-C36
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,WB
• Species Reactivity: Guinea pig
• Immunogen: Guinea Pig IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Donkey Anti-Guinea Pig IgG (H&L) Antibody Pre-Adsorbed, Donkey Anti Guinea Pig IgG
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Hamster (all),Equine,Human,Mouse,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Guinea Pig
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:25,000 - 1:75,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Guinea Pig IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

ELISA results of purified Donkey Anti-Guinea Pig IgG Antibody Min X10 Serums tested against purified Guinea Pig IgG. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (p/n 006-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Gt-a-Dky IgG HRP conjugate (p/n 616-1302), and TMB substrate (p/n TMBE-1000).