Rabbit IgG anti-Horse IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Horse IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-608-4302
• Supplier Catalog Number: 608-4302
• Alternative Catalog Number: ROC-608-4302
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Equine
• Immunogen: Anti-Horse IgG whole molecule was produced by repeated immunization with Horse IgG whole molecule in rabbit.
• Conjugation: HRPO
• Alternative Names: rabbit Anti-Horse IgG peroxidase Conjugated Antibody, rabbit Anti-Horse IgG Antibody peroxidase conjugate, rabbit Anti-Horse IgG Antibody HRP Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Horse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:50,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:5,000 - 1:20,000
• Application Notes: Anti-Horse IgG Peroxidase Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be op

Images

ELISA results of purified Rabbit anti-Horse IgG Antibody Peroxidase Conjugated tested against purified Horse IgG. Each well was coated in duplicate with 1.0 µg of Horse IgG(p/n 008-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE-1000.