Horse IgG (H&L) Antibody Alkaline Phosphatase Conjugated, ALP, Rabbit, Polyclonal

Product Overview

• Article Name: Horse IgG (H&L) Antibody Alkaline Phosphatase Conjugated, ALP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-608-4502
• Supplier Catalog Number: 608-4502
• Alternative Catalog Number: ROC-608-4502
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Equine
• Immunogen: Horse IgG whole molecule
• Conjugation: ALP
• Alternative Names: Rabbit Anti-Horse IgG alkaline phosphatase Conjugated Antibody, Rabbit Anti-Horse IgG alk phos Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Horse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:20,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Horse IgG Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-l

Images

ELISA results of purified Goat anti-Human IgG antibody Alk Phos Conjugation. Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using NPP-B500 buffer, Streptavidin HRP conjugate at 1:10,000 and NPP-10 substrate.