Goat IgG anti-Human IgG (Fc)-unconj., MinX Ms, Polyclonal , Unconjugated

Product Overview

• Article Name: Goat IgG anti-Human IgG (Fc)-unconj., MinX Ms, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-609-101-017
• Supplier Catalog Number: 609-101-017
• Alternative Catalog Number: ROC-609-101-017
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG F(c) fragment
• Conjugation: Unconjugated
• Alternative Names: Goat Anti Human IgG Fc Antibody Pre-Adsorbed, Goat Anti-Human IgG F(c) Antibody, Goat Anti Human IgG Fc Fragment Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): Mouse

Product Properties

• Clonality: Polyclonal
• Concentration: 1.1 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:15,000 - 1:60,000, IHC: 1:1,000 - 1:3,000, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Human IgG F(c) antibody has been tested by dot blot and ELISA. This antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

ELISA results of purified Goat anti-Human IgG F(c) antibody (min x Mouse serum proteins) tested against purified Human IgG F(c) (p/n 009-0103). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) as well as Mouse IgG (p/n 010-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Donkey anti-Goat IgG Antibody Peroxidase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) (p/n 605-703-125) and TMB substrate p/n TMBE-1000.