Goat IgG anti-Human IgG+IgM+IgA (H+L)-unconj., MinX none

Product Overview

• Article Name: Goat IgG anti-Human IgG+IgM+IgA (H+L)-unconj., MinX none
• Biozol Catalog Number: ROC-609-101-130
• Supplier Catalog Number: 609-101-130
• Alternative Catalog Number: ROC-609-101-130
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG, IgA and IgM whole molecules
• Conjugation: Unconjugated
• Alternative Names: Goat anti Human IgG IgA IgM Antibody, Goat Anti-Human IgG IgA IgM Antibody, Goat Anti-Human IgGAM
• Format: IgG
• Target Specificity: IgG+IgM+IgA (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:28,800 - 1:48,800, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Human IgG IgA IgM antibody has been tested by ELISA and dot blot and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

ELISA Results of purified Goat Anti-Human IgG, IgA, IgM Antibody tested against purified Human IgG, IgA, IgM. The working dilution for Anti-Human IgG, IgA, IgM is 1:20,000. Each well was coated in duplicate with 1.0 µg of Human IgG protein [Red Line], Human IgA [Green Line], Human IgM [Blue Line], Human IgG/A/M [Purple Line]. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Donkey Anti-Goat IgG HRP conjugated and TMBE-1000 substrate.