Goat IgG anti-Human IgG (Fc)-Alk. Phos., MinX Ms, ALP, Polyclonal , AP

Product Overview

• Article Name: Goat IgG anti-Human IgG (Fc)-Alk. Phos., MinX Ms, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-609-105-017
• Supplier Catalog Number: 609-105-017
• Alternative Catalog Number: ROC-609-105-017
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG F(c) fragment
• Conjugation: Alk. Phos.
• Alternative Names: goat anti-Human IgG F(c) alkaline phosphatase Conjugated Antibody, goat anti-Human IgG F(c) fragment antibody alkaline phosphatase conjugation
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): Mouse

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:1,000 - 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
• Application Notes: Anti-Human IgG F(c) Alkaline Phosphatase Conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot

Images

ELISA Results of purified Goat Anti-Human IgG F(c) Antibody Alkaline Phosphatase Conjugated (Min X Mouse serum Proteins) tested against purified Human IgG F(c) Alk Phos. Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103). The working dilution is 1:5200. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Substrate Buffer (p/n NPP-10), NPP Working Buffer (p/n NPP-B500).