Goat IgG anti-Human IgG (H+L)-HRPO, MinX Ms, Polyclonal

Product Overview

• Article Name: Goat IgG anti-Human IgG (H+L)-HRPO, MinX Ms, Polyclonal
• Biozol Catalog Number: ROC-609-1316-0100
• Supplier Catalog Number: 609-1316-0100
• Alternative Catalog Number: ROC-609-1316-0100
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: HRPO
• Alternative Names: goat anti-Human IgG peroxidase conjugated Antibody, goat anti-Human IgG Antibody HRP conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Mouse

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:250,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:20,000
• Application Notes: Anti-Human IgG (H&L) Peroxidase Conjugate has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based

Images

Diagnostic accuracy of detecting IgG and IgG/M antibodies against natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) using HRP anti-human IgG (p/n 609-1316), HRP anti-human IgM (p/n 609-4331), and HRP Protein A (p/n PA 00-03). ELISA = enzyme-linked immunosorbent assay, NPV = negative predictive value, PPV = positive predictive value. Results are shown as percent, with confidence intervals in parentheses. Table 5. PMID: 29141725.

Western Blot of Goat anti-Human IgG Pre-Adsorbed Peroxidase Conjugated Secondary Antibody. Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Human IgG. Other band(s): Human IgG splice variants and isoforms.