Goat IgG anti-Mouse IgM (µ)-HRPO, MinX none

Product Overview

• Article Name: Goat IgG anti-Mouse IgM (µ)-HRPO, MinX none
• Biozol Catalog Number: ROC-610-1307-0250
• Supplier Catalog Number: 610-1307-0250
• Alternative Catalog Number: ROC-610-1307-0250
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgM whole molecule
• Conjugation: HRPO
• Alternative Names: goat anti-Mouse IgM (Mu Chain) Peroxidase Conjugated Antibody, goat anti-Mouse IgM Mu Chain Antibody HRP Conjugation
• Format: IgG
• Target Specificity: IgM (µ)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:30,000, IHC: 1:500 - 1:2,000, WB: 1:1,000 - 1:5,000
• Application Notes: Mouse IgM (mu chain) peroxidase conjugated Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely

Images

ELISA results using Goat Anti-Mouse IgM HRP.Determination of mAb titer and affinity.(A)Titration of mAb by an indirect ELISA. The mAb was serially diluted in 1:3. The optimum working concentration was determined for a midpoint of the steep portion of the curve.(B)The measurement of antibody relative affinity by thiocyanate elution assay. The affinity index was estimated by the molarity of NH4SCN causing 50% reduction from initial absorbance in the elution curves. All experiments were carried out in triplicate and the results were calculated from three independent experiments. Figure 2. PMID: 32373546.

Immunochemical Staining using Goat Anti-Mouse IgM HRP. Identification of intact B. melitensis strain by ICS with mAbs. The intact bacteria of B. melitensis strain were stained by ICS with individual mAbs. Saturated with goat anti-mouse IgG and IgM HRP conjugate. Bacteria were visualized with diaminobenzidine (DAB) substrate for color development. G-, Gram staining for bacterial control of B. melitensis strain examined under white light with a microbiological microscope. Figure S2. PMID: 32373546.