Mouse IgG2b Antibody ATTO 532 Conjugated pre-absorbed, ATTO-532, Goat, Polyclonal

Product Overview

• Article Name: Mouse IgG2b Antibody ATTO 532 Conjugated pre-absorbed, ATTO-532, Goat, Polyclonal
• Biozol Catalog Number: ROC-610-153-042
• Supplier Catalog Number: 610-153-042
• Alternative Catalog Number: ROC-610-153-042
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: DOT
• Species Reactivity: Mouse
• Immunogen: Mouse IgG2b heavy chain
• Conjugation: ATTO-532
• Alternative Names: goat anti-MOUSE IgG2b Antibody ATTO532 Conjugation, goat anti-Mouse IgG2b (gamma 2b) ATTO 532 Conjugated Antibody
• Format: IgG
• Target Specificity: IgG2b (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: >1:20,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: >1:5,000, WB: 1:4,000 - 1:20,000
• Application Notes: Anti-Mouse IgG2b ATTO 532 Antibody has been tested by dot blot. Mouse IgG2b secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays. ATTO Dye Conjugated Secondary Antibodies are d

Images

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH

Dot Blot of Goat anti-Mouse IgG2b (Gamma 2b Chain) Antibody ATTO 532 Conjugated. Antigen: Mouse IgG2b. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Primary antibody: none. Secondary antibody: Goat anti-Mouse IgG2b (Gamma 2b Chain) Antibody ATTO 532 Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.