Goat IgG anti-Mouse IgG (H+L)-ATTO 647N, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, ATTO-647N, Polyclonal

Product Overview

• Article Name: Goat IgG anti-Mouse IgG (H+L)-ATTO 647N, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, ATTO-647N, Polyclonal
• Biozol Catalog Number: ROC-610-156-121S
• Supplier Catalog Number: 610-156-121S
• Alternative Catalog Number: ROC-610-156-121S
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: FLISA,IF,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: ATTO 647N
• Alternative Names: Goat Anti-Mouse IgG Antibody ATTO647N Conjugation, Goat Anti-Mouse IgG ATTO 647N Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
• Application Notes: Anti-Mouse IgG ATTO 647N Antibody has been tested by western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multip

Images

Western Blot of ATTO 647N conjugated Goat anti-Mouse IgG Pre-Adsorbed secondary antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 647N goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): none.

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH