Rabbit IgG anti-Mouse IgG2a (Fc)-FITC, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgG2a (Fc)-FITC, MinX none
• Biozol Catalog Number: ROC-610-4241
• Supplier Catalog Number: 610-4241
• Alternative Catalog Number: ROC-610-4241
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: FLISA,FACS,IF
• Species Reactivity: Mouse
• Immunogen: Mouse IgG2a heavy chain
• Conjugation: FITC
• Alternative Names: Rabbit Anti-Mouse IgG2a (gamma 2a chain) Antibody fluorescein Conjugated, Rabbit Anti-Mouse IgG2a Antibody FITC Conjugation
• Format: IgG
• Target Specificity: IgG2a (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Anti-Mouse IgG2a Fluorescein Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex an

Images

Western blot of Fluorescein conjugated Rabbit Anti-Mouse IgG2a (Gamma 2a chain) secondary antibody. Lane 1: Mouse IgG2a. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2a (Gamma 2a chain). Other band(s): None.

Dot Blot results of Rabbit Anti-Mouse IgG2a Antibody Fluorescein Conjugated. Dots are Mouse IgG2a at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 30 min at RT. Primary Antibody: Rabbit Anti-Mouse IgG2a FITC at 1µg/mL for 1hr at RT. Secondary Antibody: none. Imaged with BioRad ChemiDoc, FITC filter.