Mouse IgG F(ab)2 Antibody Peroxidase Conjugated Pre-Adsorbed, HRP, Rabbit, Polyclonal

Product Overview

• Article Name: Mouse IgG F(ab)2 Antibody Peroxidase Conjugated Pre-Adsorbed, HRP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-610-4321
• Supplier Catalog Number: 610-4321
• Alternative Catalog Number: ROC-610-4321
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG F(ab)2 fragment
• Conjugation: HRP
• Alternative Names: Rabbit Anti-Mouse IgG F(ab)2 Antibody peroxidase Conjugation, Rabbit Anti-Mouse IgG Fab2 Antibody peroxidase Conjugated, Rabbit Anti-Mouse IgG Fab2 Fragment HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:16,000, IHC: 1:500 - 1:2,000, WB: 1:500 - 1:2,000
• Application Notes: Anti-Mouse IgG F(ab)2 Peroxidase conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Images

Titers for anti-Gal antibodies. Serial 1:2 dilutions of serum from alpha1,3galactosyltransferase knockout mice were incubated on plates coated with alpha-gal-linked bovine serum albumin. Titers of 1:160 or greater were considered immunity comparable with human titers and appropriate for use in experimentation. Highlighted area in yellow shows titers of tested mice at 1:320 and -1:640, greater than the needed threshold. FIGURE 1. PMID: 29481481.

Titers for anti-Gal antibodies. Serial 1:2 dilutions of serum from alpha1,3galactosyltransferase knockout mice were incubated on plates coated with alpha-gal-linked bovine serum albumin. Titers of 1:160 or greater were considered immunity comparable with human titers and appropriate for use in experimentation. Supplementary Figure 1. PMID: 34348342.