Rabbit IgG anti-Mouse IgG2a (Fc)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgG2a (Fc)-HRPO, MinX none
• Biozol Catalog Number: ROC-610-4341
• Supplier Catalog Number: 610-4341
• Alternative Catalog Number: ROC-610-4341
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Anti-Mouse IgG2a heavy chain antibody was produced by repeated immunization with Mouse IgG2a heavy chain in rabbit.
• Conjugation: HRPO
• Alternative Names: Rabbit Anti-Mouse IgG2a (gamma 2a chain) Antibody peroxidase Conjugated, Rabbit Anti-Mouse IgG2a Antibody HRP Conjugation
• Format: IgG
• Target Specificity: IgG2a (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Mouse IgG2a heavy chain Peroxidase Conjugated antibody has been tested by ELISA and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Images

ELISA Results of Rabbit Anti-Mouse IgG2a Antibody Peroxidase Conjugated tested against purified Mouse IgGa HRP. Each well was coated in duplicate with 10 µg of Mouse IgG2a (p/n 010-0141). The working dilution is 1:37,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066) and TMB substrate (p/n TMBE-1000).