Rabbit IgG anti-Mouse IgG (Fc-Fragment)-ATTO 550, MinX none, ATTO-550, Polyclonal

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgG (Fc-Fragment)-ATTO 550, MinX none, ATTO-550, Polyclonal
• Biozol Catalog Number: ROC-610-454-C46S
• Supplier Catalog Number: 610-454-C46S
• Alternative Catalog Number: ROC-610-454-C46S
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: FLISA,IF,WB
• Species Reactivity: Mouse
• Immunogen: highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
• Conjugation: ATTO 550
• Alternative Names: rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO550 conjugation, rabbit anti-Mouse IgG ATTO550 conjugated Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
• Application Notes: Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 550 has been tested by western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This p

Images

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH

Western Blot of ATTO 550 Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, 3) secondary antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a. Lane 3: Mouse IgG2b. Lane 4: Mouse IgG3. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: ATTO 550 rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG Subclasses. Other band(s): None.

Atto(TM) dyes can be used for multicolor immunofluorescent detection with low background and high signal. Example shown here is Immunohistochemical staining using ATTO-550 Anti-Aquaporin 2-antibody (red) of paraffin embedded region of rat kidney showing a transversal cut of the inner medulla near to the renal papilla. Nuclei are visualized with Hoechst 33342 (blue). Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH