Rabbit IgG anti-Mouse IgG1 (Fc)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgG1 (Fc)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-610-4540
• Supplier Catalog Number: 610-4540
• Alternative Catalog Number: ROC-610-4540
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG1 heavy chain
• Conjugation: Alk. Phos.
• Alternative Names: Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody alkaline phosphatase Conjugation, Rabbit Anti-Mouse IgG1 alk phos Conjugated Antibody
• Format: IgG
• Target Specificity: IgG1 (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Mouse IgG1 Alkaline Phosphatase Antibody has been tested by ELISA, dot blot, and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.

Images

Western Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4C. Block: MB-070 for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.

Dot Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG1. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 for one hour at RT. Block: MB-070 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.