Rabbit IgG anti-Mouse IgG2a (Fc)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgG2a (Fc)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-610-4541
• Supplier Catalog Number: 610-4541
• Alternative Catalog Number: ROC-610-4541
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG2a heavy chain
• Conjugation: Alk. Phos.
• Alternative Names: Rabbit Anti-Mouse IgG2a (gamma 2a chain) Antibody alkaline phosphatase Conjugated, Rabbit Anti-Mouse IgG2a Antibody alk phos Conjugation
• Format: IgG
• Target Specificity: IgG2a (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Mouse IgG2a Alkaline Phosphatase antibody has been tested by ELISA, dot blot, and western blot This antibody is assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 mi

Images

Western Blot of Mouse IgG2a Antibody Alkaline Phosphatase Conjugated. Lane 1: Mouse IgG2a. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG2a Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4C. Block: MB-070 for 30 min at RT. Reaction visualized with alkaline phosphatase substrate for 30 seconds at RT. Predicted/Observed size: 55kDa for Mouse IgG2a.

Dot Blot of Mouse IgG2a Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG2a Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4 Lane 2.5ng Primary antibody: none Secondary antibody: Mouse IgG2a Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.