Rabbit IgG anti-Mouse IgG2a (Fc)-Biotin, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgG2a (Fc)-Biotin, MinX none
• Biozol Catalog Number: ROC-610-4641
• Supplier Catalog Number: 610-4641
• Alternative Catalog Number: ROC-610-4641
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG2a heavy chain
• Conjugation: Biotin
• Alternative Names: Rabbit Anti-Mouse IgG2a (Gamma 2a chain) Antibody Biotin Conjugated, Rabbit Anti Mouse IgG2a Biotin Conjugated Antibody
• Format: IgG
• Target Specificity: IgG2a (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Mouse IgG2a Biotin Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

Images

ELISA Results of Rabbit Anti-Mouse IgG2a Antibody Biotin Conjugated tested against purified Mouse IgG2a Biotin. Each well was coated in duplicate with 1.0 µg of Mouse IgG2a (p/n 010-0141). The working dilution is 1:81,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03) and TMB substrate (p/n TMBE-1000).