Sheep IgG anti-Mouse IgG (H+L)-FITC, MinX none

Product Overview

• Article Name: Sheep IgG anti-Mouse IgG (H+L)-FITC, MinX none
• Biozol Catalog Number: ROC-610-602-002
• Supplier Catalog Number: 610-602-002
• Alternative Catalog Number: ROC-610-602-002
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: FLISA,FACS,IF
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: FITC
• Alternative Names: sheep anti-Mouse IgG fluorescein Conjugated Antibody, sheep anti-Mouse IgG Antibody FITC Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Anti-Mouse IgG Fluorescein Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex anal

Images

Western Blot of Fluorescein Sheep Anti-Mouse IgG secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein sheep secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.