Donkey IgG anti-Mouse IgG (H+L)-unconj., MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, Polyclonal , Unconjugated

Product Overview

• Article Name: Donkey IgG anti-Mouse IgG (H+L)-unconj., MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-610-701-124
• Supplier Catalog Number: 610-701-124
• Alternative Catalog Number: ROC-610-701-124
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.
• Conjugation: Unconjugated
• Alternative Names: Donkey anti-Mouse IgG Antibody, Donkey anti-Mouse IgG pre-adsorbed Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Suitable for immunoblotting (western or dot blot), ELISA, electron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Images

Western blot assay of L1 and SEMA 3A protein expressions at the proximal end (A) and (B) distal end of injured sensory and motor nerves.(A) Bands of L1 and SEMA 3A protein expressions at the proximal end of the injured nerves. (B) Bands of L1 and SEMA 3A protein expressions at the distal end of injured nerves.Primary antibody mouse monoclonal anti-neural cell adhesion molecule L1 (1:500), rabbit polyclonal anti-semaphorin 3A (1:1,000), and mouse monoclonal to beta-actin (1:10,000) at 4C overnight. Secondary antibody anti-rabbit IgG (H&L) (DONKEY) antibody (1:5,000) or anti-mouse IgG (H&L) (DONKEY) antibody (1:5,000) at room temperature for 1.5 hours.Experiments were performed in triplicate. MN: Motor nerves, SN: sensory nerves, L1: nerve cell adhesion molecule L1, SEMA 3A: semaphorin 3A. Fig 3A, Fig 4A. PMID: 28197202.