Rabbit IgG Fc Antibody Biotin Conjugated Pre-Adsorbed, Goat, Polyclonal

Product Overview

• Article Name: Rabbit IgG Fc Antibody Biotin Conjugated Pre-Adsorbed, Goat, Polyclonal
• Biozol Catalog Number: ROC-611-1623
• Supplier Catalog Number: 611-1623
• Alternative Catalog Number: ROC-611-1623
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG F(c) fragment
• Conjugation: Biotin
• Alternative Names: Goat anti-Rabbit IgG F(c) Antibody biotin Conjugation, Goat anti-Rabbit IgG Fc fragment Antibody biotin Conjugation, Goat anti-Rabbit IgG F(c) BAC Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-RABBIT IgG F(c) (GOAT) Biotin antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot

Images

Western Blot of Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103) 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.