Rat IgG anti-Rabbit IgG (H+L)-unconj., MinX Hu,Go,Ms, Polyclonal , Unconjugated

Product Overview

• Article Name: Rat IgG anti-Rabbit IgG (H+L)-unconj., MinX Hu,Go,Ms, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-611-501-122
• Supplier Catalog Number: 611-501-122
• Alternative Catalog Number: ROC-611-501-122
• Manufacturer: Rockland Immunochemicals
• Host: Rat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Rat Anti-Rabbit IgG Antibody Pre-Adsorbed, Rat Anti-Rabbit IgG (H&L) Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Human,Goat,Mouse

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:900,000, IHC: 1:1,000 - 1:5,000, WB: 1:20,000 - 1:100,000
• Application Notes: Anti-Rabbit IgG antibody has been tested by ELISA and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of Anti-Rabbit IgG (H&L) (RAT) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-501-122). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (RAT) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-501-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Rat IgG (RABBIT) Peroxidase Conjugated Antibody (p/n 612-4302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.