Rat IgG anti-Rabbit IgG (H+L)-Biotin, MinX Hu,Go,Ms, Polyclonal

Product Overview

• Article Name: Rat IgG anti-Rabbit IgG (H+L)-Biotin, MinX Hu,Go,Ms, Polyclonal
• Biozol Catalog Number: ROC-611-506-122
• Supplier Catalog Number: 611-506-122
• Alternative Catalog Number: ROC-611-506-122
• Manufacturer: Rockland Immunochemicals
• Host: Rat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Rat Anti-Rabbit IgG Biotin Conjugated Antibody, Rat Anti-Rabbit IgG Antibody Biotin Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Human,Goat,Mouse

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Rabbit IgG Biotin Conjugated Antibody has been has been tested by ELISA and is assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfon

Images

ELISA Results of Rat Anti-Rabbit IgG Antibody Biotin Conjugated (Min X Hu Gt Ms Serum Proteins) tested against purified Rabbit IgG MX3 Biotin. Each well was coated in duplicate with 1.0 µg of Rabbit IgG (p/n 011-0102). The working dilution is 1:62,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03), and TMB substrate (p/n TMBE-1000).