Rabbit IgG (H&L) Antibody Fluorescein Conjugated, FITC, Sheep, Polyclonal

Product Overview

• Article Name: Rabbit IgG (H&L) Antibody Fluorescein Conjugated, FITC, Sheep, Polyclonal
• Biozol Catalog Number: ROC-611-6202
• Supplier Catalog Number: 611-6202
• Alternative Catalog Number: ROC-611-6202
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: DOT
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Conjugation: FITC
• Alternative Names: sheep anti-Rabbit IgG Antibody fluorescein Conjugation, sheep anti-Rabbit IgG FITC Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Anti-Rabbit IgG Antibody fluorescein conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis,

Images

Western Blot of Anti-Rabbit IgG (H&L) (SHEEP) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-6102). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (SHEEP) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-6102) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody (p/n 613-703-168) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.