Rat IgM (mu chain) Antibody Alkaline Phosphatase Conjugated, ALP, Goat, Polyclonal

Product Overview

• Article Name: Rat IgM (mu chain) Antibody Alkaline Phosphatase Conjugated, ALP, Goat, Polyclonal
• Biozol Catalog Number: ROC-612-1507
• Supplier Catalog Number: 612-1507
• Alternative Catalog Number: ROC-612-1507
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: DOT, ELISA
• Species Reactivity: Rat
• Immunogen: Rat IgM whole molecule
• Conjugation: ALP
• Alternative Names: goat anti-Rat IgM (mu chain) Antibody Alkaline Phosphatase Conjugation, goat anti-Rat IgM mu chain Alkaline Phosphatase Conjugated Antibody
• Format: IgG
• Target Specificity: IgM (µ)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:30,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Rat IgM Alkaline Phosphatase antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Rat IgM in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature. A wo

Images

Western Blot of Anti-Rat IgM (mu chain) (GOAT) Antibody (p/n 612-1107). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody (p/n 612-1107) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.