Rat IgG Fc Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Rat IgG Fc Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-612-4103
• Supplier Catalog Number: 612-4103
• Alternative Catalog Number: ROC-612-4103
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Rat
• Immunogen: Rat IgG F(c) fragment
• Conjugation: Unconjugated
• Alternative Names: Rabbit Anti Rat IgG F(c) Antibody, Rabbit Anti-Rat IgG Fc Fragment Antibody, Rabbit Anti-Rat IgG Fc Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.1 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Rat IgG F(c) antibody is tested by western blot and is suitable for ELISA and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

Western Blot of Anti-Rat IgG F(c) (RABBIT) Antibody (p/n 612-4103). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG F(c) (RABBIT) Antibody (p/n 612-4103) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.