Rabbit IgG anti-Swine IgG (F(ab)2)-unconj., MinX none

Product Overview

• Article Name: Rabbit IgG anti-Swine IgG (F(ab)2)-unconj., MinX none
• Biozol Catalog Number: ROC-614-4104
• Supplier Catalog Number: 614-4104
• Alternative Catalog Number: ROC-614-4104
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Porcine
• Immunogen: Swine IgG F(ab)2 fragment
• Conjugation: Unconjugated
• Alternative Names: Rabbit Anti Swine IgG F(ab)2 Antibody, Rabbit Anti-Swine IgG Fab2 Antibody, Rabbit Anti-Swine IgG Fab2 Fragment Antibody
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 4.6 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Swine
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Swine IgG F(ab)2 antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Images

ELISA Results of Rabbit Anti-Swine IgG F(ab)2 Antibody tested against purified Swine IgG F(ab)2. Each well was coated in duplicate with 1.0 µg of Swine IgG F(ab)2 (p/n 014-0104) [Green Line] and Swine IgG (p/n 014-0102) [Blue Line]. The working dilution of Swine IgG F(ab)2 is 1:88,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Goat Anti-Rabbit IgG HRP conjugated (p/n 611-103-122) and TMB substrate (p/n TMBE-1000).