Swine IgG F(ab)2 Antibody Alkaline Phosphatase Conjugated, ALP, Rabbit, Polyclonal

Product Overview

• Article Name: Swine IgG F(ab)2 Antibody Alkaline Phosphatase Conjugated, ALP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-614-4504
• Supplier Catalog Number: 614-4504
• Alternative Catalog Number: ROC-614-4504
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Porcine
• Immunogen: Swine IgG F(ab)2 fragment
• Conjugation: ALP
• Alternative Names: Rabbit Anti-Swine IgG F(ab)2 alkaline phosphatase Conjugated Antibody, Rabbit Anti-Swine IgG Fab2 alkaline phosphatase Conjugated Antibody, Rabbit Anti-Swine IgG Fab2 Fragment Antibody alk phos Conjugation
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Swine
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:1,000 - 1:3,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
• Application Notes: Anti-Swine IgG F(ab)2 alkaline phosphatase conjugated antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Images

ELISA Results of Rabbit Anti-Swine IgG F(ab)2 Antibody Alkaline Phosphatase Conjugate tested against purified Swine IgG F(ab)2 Alk Phos. Each well was coated in duplicate with 1.0 µg of Swine IgG (p/n 014-0102). The working dilution is 1:37,500. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Substrate Buffer (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).