Rabbit IgG anti-Llama IgG2a (Fc)-unconj., MinX none

Product Overview

• Article Name: Rabbit IgG anti-Llama IgG2a (Fc)-unconj., MinX none
• Biozol Catalog Number: ROC-634-401-044
• Supplier Catalog Number: 634-401-044
• Alternative Catalog Number: ROC-634-401-044
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Llama
• Immunogen: Llama IgG2
• Conjugation: Unconjugated
• Alternative Names: IgG2 antibody, Llama IgG2, anti-subclass secondary antibody
• Format: IgG
• Target Specificity: IgG2a (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.07 mg/ml by UV absorbance at 280 nm
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Llama
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:60,0000, IHC: User Optimized, WB: 1:20,000-1:40,000
• Application Notes: Antibody Anti-Llama IgG2 has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.

Images

Western Blot of Rabbit Anti-Llama IgG2 antibody. Lane 1: Opal Pre-stained Ladder (MB-210-0500). Lane 2: Llama IgG1 (p/n 025-0142). Lane 3: Llama IgG2 (p/n 025-0143). Load: 50 ng per lane. Product: Rabbit Anti-Llama IgG2 at 1:40,000. Secondary antibody: Gt-a-Rb HRP (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) overnight at 4C. Predicted/Observed size: 48 kDa for Llama IgG2. Other band(s): ~38 kda non-specific. Non-specific background against IgG1 (HC) can be reduced by further diluting the antibody. User optimization recommended.