Donkey F(ab)2 anti-Goat IgG (H+L)-unconj., MinX Ck,Gp,Hm,Ho,Hu,Ms,Rb,Rt, Polyclonal , Unconjugated

Product Overview

• Article Name: Donkey F(ab)2 anti-Goat IgG (H+L)-unconj., MinX Ck,Gp,Hm,Ho,Hu,Ms,Rb,Rt, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-705-701-125
• Supplier Catalog Number: 705-701-125
• Alternative Catalog Number: ROC-705-701-125
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Goat
• Immunogen: Goat IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Donkey F(ab)2 Anti-Goat IgG Antibody, Donkey Fab2 Anti-Goat IgG Antibody
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Gallus,Guinea pig,Hamster (all),Equine,Human,Mouse,Rabbit,Rat

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Goat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 Anti-Goat IgG Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated bind

Images

Dot Blot Results of F(ab)2 Donkey Anti-GOAT IgG [H&L] Antibody Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, Rt Serum Proteins. Goat IgG (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: F(ab)2 Dk-a-Gt IgG Mx8 at 1µg/mL for 1hr at RT. Secondary Antibody: Goat Anti-Donkey HRP at 1:40,000 for 30 mins at RT. Block: BlockOut Buffer (p/n MB-073) for 30 mins at RT. Exposure: 1 sec.

SDS PAGE Results of F(ab)2 Anti-GOAT IgG (DONKEY) Antibody. Lane 1: Fab2 Dk-a-Gt IgG Mx8 Reduced [1.0µg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Fab2 Dk-a-Gt IgG Mx8 Non-Reduced [1.0µg]. 4-20% Gel, Coomassie Stained.