Donkey F(ab)2 anti-Goat IgG (H+L)-HRPO, MinX Ck,Gp,Hm,Ho,Hu,Ms,Rb,Rt, Polyclonal

Product Overview

• Article Name: Donkey F(ab)2 anti-Goat IgG (H+L)-HRPO, MinX Ck,Gp,Hm,Ho,Hu,Ms,Rb,Rt, Polyclonal
• Biozol Catalog Number: ROC-705-703-125
• Supplier Catalog Number: 705-703-125
• Alternative Catalog Number: ROC-705-703-125
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Goat
• Immunogen: Goat IgG whole molecule
• Conjugation: HRPO
• Alternative Names: Donkey F(ab)2 Anti-Goat IgG Antibody Peroxidase Conjugation, Donkey Fab2 Anti-Goat IgG HRP Conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Gallus,Guinea pig,Hamster (all),Equine,Human,Mouse,Rabbit,Rat

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Goat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 Anti-Goat IgG Peroxidase Antibody has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including

Images

ELISA Results of F(ab)2 Donkey Anti-Goat IgG Peroxidase Conjugated Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, Rt Serum Proteins. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n 005-0102). The working dilution is 267,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS (p/n MB-066), and TMB substrate (p/n TMBE-1000).