Donkey F(ab)2 anti-Goat IgG (H+L)-RPE, MinX Ck,Gp,Hm,Ho,Hu,Ms,Rb,Rt, Polyclonal

Product Overview

• Article Name: Donkey F(ab)2 anti-Goat IgG (H+L)-RPE, MinX Ck,Gp,Hm,Ho,Hu,Ms,Rb,Rt, Polyclonal
• Biozol Catalog Number: ROC-705-708-125
• Supplier Catalog Number: 705-708-125
• Alternative Catalog Number: ROC-705-708-125
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: FLISA,FACS,IF,WB
• Species Reactivity: Goat
• Immunogen: Anti-Goat IgG was produced by repeated immunization with goat IgG whole molecule in donkey.
• Conjugation: RPE
• Alternative Names: Donkey F(ab)2 Anti-Goat IgG Antibody Phycoerythrin Conjugation, Donkey Fab2 Anti-Goat IgG PE Conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Gallus,Guinea pig,Hamster (all),Equine,Human,Mouse,Rabbit,Rat

Product Properties

• Clonality: Polyclonal
• Concentration: 1.032 mg/ml by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Goat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: User Optimized, Flow Cytometry: 1:100 - 1:250, IF Microscopy: 1:100 - 1:250, WB: User Optimized
• Application Notes: F(ab)2 Anti-Goat IgG Phycoerythrin Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels

Images

Dot Blot results of Donkey F(ab)2 Anti-Goat IgG Antibody Phycoerythrin Conjugated. Dots are Goat IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 30 min at RT. Primary Antibody: none. Secondary Antibody: Donkey F(ab)2 Anti-Goat IgG Antibody RPE at 1µg/mL for 1hr at RT. Imaged with BioRad ChemiDoc, Rhodamine filter.

Dot Blot of F(ab)2 Donkey anti-Goat IgG Phycoerythrin Conjugated Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, & Rt serum proteins antibody. Antigen: Goat IgG. Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng, or 1.23 ng as indicated. Primary antibody: N/A. Secondary antibody: F(ab)2 Donkey anti-Goat IgG Phycoerythrin Conjugated Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, & Rt serum proteins antibody at 1:1,000 for 60 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 60 min at RT.