Goat F(ab)2 anti-Human IgG (Fc)-HRPO, MinX Bo,Ho,Ms,Rt, Polyclonal

Product Overview

• Article Name: Goat F(ab)2 anti-Human IgG (Fc)-HRPO, MinX Bo,Ho,Ms,Rt, Polyclonal
• Biozol Catalog Number: ROC-709-1317
• Supplier Catalog Number: 709-1317
• Alternative Catalog Number: ROC-709-1317
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG F(c) fragment
• Conjugation: HRPO
• Alternative Names: Goat F(ab)2 Anti-Human IgG F(c) Peroxidase Conjugated Pre-Adsorbed Antibody, Goat Fab2 Anti-Human IgG Fc Fragment Peroxidase Conjugated Antibody, Goat F(ab)2 Anti-Human IgG F(c) Fragment Antibody HRP Conjugated
• Format: F(ab')2
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): Bovine,Equine,Mouse,Rat

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:130,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: F(ab)2 Anti-Human IgG F(c) Peroxidase Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection

Images

ELISA results of purified F(ab)2 Goat anti-Human IgG F(c) Antibody Peroxidase Conjugated min x Bv, Hs, Ms, ant Rt serum proteins tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE-1000.