Goat F(ab)2 anti-Human Kappa light chain-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Goat F(ab)2 anti-Human Kappa light chain-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-709-1510
• Supplier Catalog Number: 709-1510
• Alternative Catalog Number: ROC-709-1510
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human kappa light chain
• Conjugation: Alk. Phos.
• Alternative Names: Goat F(ab)2 Anti-Human kappa (kappa chain) Antibody Alkaline Phosphatase Conjugation, Goat F(ab)2 Anti-Human kappa chain alk phos Conjugated Antibody
• Format: F(ab')2
• Target Specificity: Kappa (light chain)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution conc

Images

Standard curves generated using the respective human IgG and IgCw-gammakappa. ELISA data from the right-hand panels were used to plot the log graphs. Human IgG and IgCw-gamma with known concentrations were placed in wells coated with goat anti-human IgG/Fc antibody as a capture antibody, and bound Ig molecules were detected with AP-conjugated goat anti-human Ckappa antibody F (ab)2as a detection antibody. Data are presented as meanSD, n=3. Fig.4.PMID: 30910358.