Goat F(ab)2 anti-Human IgG (F(ab)2)-Alk. Phos., MinX Bo,Ho,Ms,Rt, ALP, Polyclonal , AP

Product Overview

• Article Name: Goat F(ab)2 anti-Human IgG (F(ab)2)-Alk. Phos., MinX Bo,Ho,Ms,Rt, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-709-1518
• Supplier Catalog Number: 709-1518
• Alternative Catalog Number: ROC-709-1518
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG F(ab)2 fragment
• Conjugation: Alk. Phos.
• Alternative Names: Goat F(ab)2 Anti-Human IgG F(ab)2 Alkaline Phosphatase Conjugated Antibody Pre-Adsorbed, Goat F(ab)2 Anti-Human IgG F(ab)2 Antibody alk phos Conjugation
• Format: F(ab')2
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): Bovine,Equine,Mouse,Rat

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:20,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: F(ab)2 Anti-Human IgG F(ab)2 Alkaline Phosphatase Antibody has been tested by ELISA and is assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature.

Images

ELISA Results of Goat F(ab)2 Anti-Human IgG F(ab)2 MX4 Antibody Alkaline Phosphatase Conjugated. Each well was coated in duplicate with 10 µg of Human IgG F(ab)2 (p/n 009-0104). The working dilution is 56100. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS (p/n MB-066) and TMB substrate (p/n TMBE-1000).