F(ab)2 Mouse IgG Fc Antibody Peroxidase Conjugated Pre-Adsorbed, HRP, Goat, Polyclonal

Product Overview

• Article Name: F(ab)2 Mouse IgG Fc Antibody Peroxidase Conjugated Pre-Adsorbed, HRP, Goat, Polyclonal
• Biozol Catalog Number: ROC-710-1322
• Supplier Catalog Number: 710-1322
• Alternative Catalog Number: ROC-710-1322
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Mouse
• Immunogen: Mouse IgG F(c) fragment
• Conjugation: HRP
• Alternative Names: Goat F(ab)2 Anti-Mouse IgG F(c) Antibody Peroxidase Conjugation, Goat Fab2 Anti-Mouse IgG Fc HRP Conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): Bovine,Equine,Human

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:100,000, IHC: 1:250 - 1:1000, WB: 1:500 - 1:2,000
• Application Notes: F(ab)2 Anti-Mouse IgG F(c) Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated b

Images

ELISA Results of F(ab)2 Goat Anti-Mouse IgG Fc Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Mouse IgG Fc (p/n 010-0103). The working dilution is 171,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS (p/n MB-066) and TMB substrate (p/n TMBE-1000).