Goat F(ab)2 anti-Mouse IgG (Fc)-Alk. Phos., MinX Bo,Ho,Hu, ALP, Polyclonal , AP

Product Overview

• Article Name: Goat F(ab)2 anti-Mouse IgG (Fc)-Alk. Phos., MinX Bo,Ho,Hu, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-710-1522
• Supplier Catalog Number: 710-1522
• Alternative Catalog Number: ROC-710-1522
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG F(c) fragment
• Conjugation: Alk. Phos.
• Alternative Names: Goat F(ab)2 Anti-Mouse IgG F(c) Antibody Alkaline Phosphatase Conjugation, Goat Fab2 Anti-Mouse IgG Fc alk phos Conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): Bovine,Equine,Human

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Fab)2 Anti-Mouse IgG F(c) Alkaline Phosphatase Antibody has been tested by ELISA and is assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature. A

Images

ELISA Results of F(ab,)2 Goat Anti-Mouse IgG F(c) Antibody Alkaline Phosphatase Conjugated. Each well was coated in duplicate with 10 µg of Mouse IgG F(c) (p/n 010-0103). The working dilution is 40,700. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS (p/n MB-066), NPP ELISA Alkaline Phosphatase Substrate (NPP-10), and TMB substrate (p/n TMBE-1000).