F(ab)2 Mouse IgG Fc Antibody Biotin Conjugated Pre-Adsorbed, Goat, Polyclonal

Product Overview

• Article Name: F(ab)2 Mouse IgG Fc Antibody Biotin Conjugated Pre-Adsorbed, Goat, Polyclonal
• Biozol Catalog Number: ROC-710-1622
• Supplier Catalog Number: 710-1622
• Alternative Catalog Number: ROC-710-1622
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Mouse
• Immunogen: Mouse IgG F(c) fragment
• Conjugation: Biotin
• Alternative Names: Goat F(ab)2 Anti-Mouse IgG F(c) Antibody Biotin Conjugation, Goat Fab2 Anti-Mouse IgG Fc Biotin Conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): Bovine,Equine,Human

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 Anti-Mouse IgG F(c) Biotin Antibody has been tested by ELISA and is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])

Images

ELISA results of purified Goat F(ab)2 Anti-MOUSE IgG F(c) Antibody Biotin Conjugated Min X Bv Hs & Hu Serum Proteins tested against purified Mouse IgG F(c) (Green Line). Each well was coated in duplicate with 1.0 µg of Mouse IgG F(c). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Streptavidin-HRP conjugated (p/n S000-03), and TMB substrate (p/n TMBE-1000).