Donkey F(ab)2 anti-Mouse IgG (H+L)-RPE, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, Polyclonal

Product Overview

• Article Name: Donkey F(ab)2 anti-Mouse IgG (H+L)-RPE, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-710-708-124
• Supplier Catalog Number: 710-708-124
• Alternative Catalog Number: ROC-710-708-124
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: FACS,IF
• Species Reactivity: Mouse
• Immunogen: F(ab)2 Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.
• Conjugation: RPE
• Alternative Names: Donkey F(ab)2 Anti-Mouse IgG Antibody Phycoerythrin Conjugation, Donkey Fab2 Anti-Mouse IgG PE Conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/mL by absorbance = 82.0 at 565 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: Flow Cytometry: 1:100 - 1:250, IF Microscopy: 1:100 - 1:250
• Application Notes: F(ab)2 Anti-Mouse IgG Phycoerythrin has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediate

Images

Dot Blot of F(ab)2 Donkey Anti-Mouse IgG Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins. Antigen: Mouse IgG. Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng or 1.23 ng as indicated. Primary antibody: N/A. Secondary antibody: F(ab)2 Donkey Anti-Mouse IgG Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins at 1:1,000 for 60 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 60 min at RT.

Dot Blot results of Donkey F(ab)2 Anti-Mouse IgG Antibody Phycoerythrin Conjugated. Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: Donkey F(ab)2 Anti-Mouse IgG Antibody Phycoerythrin at 1µg/mL for 1hr at RT. Secondary Antibody: none. Imaged with BioRad ChemiDoc, Rhodamine filter.