Goat F(ab)2 anti-Rabbit IgG (H+L)-FITC, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal

Product Overview

• Article Name: Goat F(ab)2 anti-Rabbit IgG (H+L)-FITC, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-711-1222
• Supplier Catalog Number: 711-1222
• Alternative Catalog Number: ROC-711-1222
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: FLISA,FACS,IF
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Conjugation: FITC
• Alternative Names: Goat F(ab)2 Anti-Rabbit IgG Fluorescein Conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody FITC Conjugation
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: F(ab)2 Anti-Rabbit IgG Fluorescein Conjugated Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable

Images

Dot Blot of Goat Fab2 anti-Rabbit IgG Antibody Fluorescein Conjugated Pre-Absorbed. Antigen: Rabbit IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat Fab2 anti-Rabbit IgG Antibody Fluorescein Conjugated Pre-Absorbed at 1:1,000 for 1 HR at RT. Block: MB-070 for 1 HR at RT.

Western blot of Fluorescein conjugated Goat F(ab)2 Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.