Goat F(ab)2 anti-Rabbit IgG (H+L)-HRPO, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal

Product Overview

• Article Name: Goat F(ab)2 anti-Rabbit IgG (H+L)-HRPO, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-711-1322
• Supplier Catalog Number: 711-1322
• Alternative Catalog Number: ROC-711-1322
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rabbit
• Immunogen: Anti-Rabbit IgG was produced by repeated immunization with Rabbit IgG whole molecule in goat.
• Conjugation: HRPO
• Alternative Names: Goat F(ab)2 Anti-Rabbit IgG Peroxidase Conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody HRP Conjugation
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:115,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 Rabbit IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(

Images

ELISA results of purified Goat F(ab)2 Anti-Rabbit IgG Antibody Peroxidase Conjugated Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins tested against purified Rabbit IgG. Each well was coated in duplicate with 1.0 µg of Rabbit IgG. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000) and TMB substrate (p/n TMBE-1000).