Goat F(ab)2 anti-Rat IgG (H+L)-unconj., MinX Ms, Polyclonal , Unconjugated

Product Overview

• Article Name: Goat F(ab)2 anti-Rat IgG (H+L)-unconj., MinX Ms, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-712-101-033
• Supplier Catalog Number: 712-101-033
• Alternative Catalog Number: ROC-712-101-033
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rat
• Immunogen: Anti-Rat IgG was produced by repeated immunization with Rat IgG whole molecule in goat.
• Conjugation: Unconjugated
• Alternative Names: Goat F(ab)2 Anti-Rat IgG Antibody, Goat Fab2 Anti-Rat IgG Antibody
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Mouse

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Rat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 Rat IgG (H&L) Antibody has been tested by ELISA and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c)

Images

Western Blot of Goat anti-Rat IgG Antibody. Lane 1: Rat IgG (p/n 012-0102). Load: 50 ng per lane. Primary antibody: F(ab)2 Rat IgG antibody at 1,000 overnight at 4C. Secondary antibody: DyLight(TM) 649 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rat IgG.